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. 2016 Nov 18;7:1834. doi: 10.3389/fmicb.2016.01834

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Copyright © 2016 Wang, Wang, Xu and Ye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection and confirmation of Shigella-MCDA products. (A) Amplification products of Shigella-MCDA assay were visually detected by observation of the color change: tube 1, positive amplification of Shigella flexneri strain (ICDC-NPS001); negative control of Listeria monocytogenes strain (ATCC19114); negative control of Salmonella strain (ICDC-NPSa001); blank control (DW). (B) Agarose gel electrophoresis of Shigella-MCDA products was shown: lane M, DNA marker DL100; lane 1, Shigella-MCDA products of Shigella flexneris (ICDC-NPS001); lane 2, negative control (Listeria monocytogenes, ATCC19114); negative control (Salmonella, ICDC-NPSa001); lane 4, blank control (DW); (C) Lateral flow biosensor applied for visual detection of Shigella MCDA products: strip 1, positive amplification of Shigella flexneris (ICDC-NPS001); strip 2, negative control (Listeria monocytogenes, ATCC19114); strip 3, negative control (Salmonella, ICDC-NPSa001); strip 4, blank control (DW).