FIGURE 4.

The optimal amplification temperature for Shigella-MCDA primer sets. The standard MCDA reactions for detection of Shigella were monitored by real-time measurement of turbidity and the corresponding curves of concentrations of DNA were marked in the figures. The threshold value was 0.1 and the turbidity of >0.1 was considered to be positive. Eight kinetic graphs (A–H) were generated at various temperatures (60–67°C, 1°C intervals) with target pathogens DNA at the level of 10 pg per reaction. The graphs from (D–H) showed robust amplification.