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. 2016 Nov 18;7:1834. doi: 10.3389/fmicb.2016.01834

FIGURE 5.

FIGURE 5

Analytical sensitivity of Shigella-MCDA assay using serially diluted genomic DNA with Shigella flexneris strain ICDC-NPs001. Five monitoring techniques, including real time turbidity (A), colorimetric indicator (FD) (B), gel electrophoresis (C), real time fluorescence (D) and lateral flow biosensor (E), were applied for analyzing the amplification products. The serial dilutions (10 ng, 10 pg, 10 fg, 1 fg, and 0.1 fg) of target templates were subjected to standard MCDA or ET-MCDA reactions. Turbidity signals (A)/Tubes (B)/Lanes (C), Fluorescence signals (D)/Strips (E) 1–8 represented the DNA levels of 10 ng, 10 pg, 10 fg, 1 fg, and 0.1 fg per reaction, negative control (10 pg of Listeria monocytogenes genomic DNA), negative control (10 pg of Salmonella genomic DNA) and blank control (DW). The genomic DNA levels of 10 ng, 10 pg, and 10 fg per reaction produced the positive reactions.