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. 2016 Nov 18;7:1834. doi: 10.3389/fmicb.2016.01834

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Copyright © 2016 Wang, Wang, Xu and Ye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The optimal duration of time required for MCDA-LFB assay. Four different reaction times (A, 10 min; B, 15 min; C, 20 min; D, 25 min) were examined and compared at 65°C. Strips 1, 2, 3, 4, 5, 6, 7, and 8 represent DNA levels of 10 ng of Shigella templates, 10 pg of Shigella templates, 10 fg of Shigella templates, 1 fg of Shigella templates, 0.1 fg Shigella templates per tube, negative control (L. monocytogenes, 10 pg per reaction), negative control (Salmonella, 10 pg per reaction) and blank control (DW). The best sensitivity was seen when the amplification lasted for 20 min (C).