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. 2016 Nov 18;7:1834. doi: 10.3389/fmicb.2016.01834

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Copyright © 2016 Wang, Wang, Xu and Ye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analytical sensitivity of MCDA-LFB for detecting target pathogens in artificially contaminated fecal samples. Five monitoring techniques, including lateral flow biosensor (A), real time turbidity (B), real time fluorescence (C), gel electrophoresis (D), and colorimetric indicator (FD) (E), were applied for analyzing the amplification products. The serial dilutions of target templates were subjected to standard MCDA or ET-MCDA reactions. Strips (A)/Turbidity signals (B)/Fluorescence signals (C)/Lanes (D)/Tubes (E) 1–8 represented the DNA levels of 5860 CFU, 586 CFU, 58.6 CFU, 5.86 CFU, 0.586 CFU and 0.0586 CFU per reaction, negative control (non-contaminated fecal sample) and blank control (DW). The genomic DNA levels of 5860 CFU, 586 CFU, 58.6 CFU and 5.86 CFU, per reaction produced the positive reactions.