Table 1.
The primers used in this study.
| Primers namea | Sequences and modificationsb | Lengthc | Gene |
|---|---|---|---|
| Shi-F1 | 5′-ACACCTTTTCCGCGTTCC-3′ | 18 nt | ipaH |
| Shi-F2 | 5′-TGATGGACCAGGAGGGTT-3′ | 18 nt | |
| Shi-CP1 | 5′-GCGACCTGTTCACGGAATCCG-TTGACCGCCTTTCCGATAC-3′ | 40 mer | |
| Shi-CP1∗ | 5′-FITC-GCGACCTGTTCACGGAATCCG-TTGACCGCCTTTCCGATAC-3′ | 40 mer | |
| Shi-E-CP1 | 5′-Hex-TGCAATG-GCGACCT(BHQ1)GTTCACGGAATCCG-TTGACCGCCTTTCCGATAC-3′ | 47 mer | |
| Shi-CP2 | 5′-GCAGTCTTTCGCTGTTGCTGC-CCGGAGATTGTTCCATGTGA-3′ | 41 mer | |
| Shi-C1 | 5′-GCGACCTGTTCACGGAATCCG-3′ | 21 nt | |
| Shi-C2 | 5′-GCAGTCTTTCGCTGTTGCTGC-3′ | 21 nt | |
| Shi-D1 | 5′-GGTATTGCGTGCAGAGACG-3′ | 19 nt | |
| Shi-D1∗ | 5′-Biotin-GGTATTGCGTGCAGAGACG-3 | 19 nt | |
| Shi-D2 | 5′-TGATGCCACTGAGAGCTGT-3′ | 19 nt | |
| Shi-R1 | 5′-CTGAGTTTTTCCAGCCATGCA-3′ | 21 nt | |
| Shi-R2 | 5′-TGCCTCTGCGGAGCTTCG-3′ | 18 nt |
aShi, shigella; Shi-CP1∗, 5′-labeled with FITC when used in MCDA-LFB assay; Shi-D1∗, 5′-labeled with biotin when used in MCDA-LFB assay; Shi-E-CP1, 5′-labeled with Hex when used in ET-MCDA assay; bHex, hexachloro-fluorescein; FITC, fluorescein isothiocyanate. cmer, monomeric unit; nt, nucleitide.