Figure 4.

Light-microscope appearance of a transgenic Tg(fli1a:eGFP) larva at 48 hpi after intravenous injection of live bacteria (A), showing an impaired blood flow ending in an almost completely stopped circulation, accompanied by pericardial oedema formation (arrow). Section view of a CLSM acquired 3D stack (B) after IF staining with an anti-Waddlia antibody, showing the infection of GFP expressing endothelial cells (green) with W. chondrophila (red). Host cell nuclei were stained with DAPI (blue). Surface rendering of the tail artery and caudal vein shows the distribution of the inclusions inside the vasculature at 24 hpi (C) and 36 hpi (D), showing bacterial spread across endothelial bounds (arrows).