Figure 7.

Survival rates (A) and bacterial load (B) are shown for larvae after intravenous injection of live W. chondrophila and subsequent treatment with 30 μg/ml tetracycline (tet) or 30 μg/ml ampicillin (amp) or left untreated (unt). Another group was injected with heat inactivated W. chondrophila (heat inact) for comparison. For survival rates (A) 10 larvae of each condition were observed in three independent experiments, respectively. The bacterial load (B) was determined at 36 hpi by qPCR of total DNA extracts from homogenates of individual larvae, targeting the W. chondrophila 16S rRNA sequence. Statistical analysis was done by one-way ANOVA with Bonferroni's posttest. ^***p < 0.001, ^**p < 0.01, ns = not significant. Mean values ± SEM are shown by horizontal bars.