Fig. 2.

Subcellular localization patterns of GFP-AtNACK1 during the formation of cell plates in BY-2 cells. BY-2 cells transformed with estradiol-inducible GFP-AtNACK1 were incubated for 16 h in the presence of 0.1 µM 17-β-estradiol. Living cells expressing GFP-AtNACK1 were observed by confocal fluorescence microscopy to detect fluorescence of sGFP (upper) and recorded as time-lapse micrographs at the indicated times during expansion of the cell plate. Normarski (DIC) and merged images (merged) are shown in the center and lower panels, respectively. Bar 10 µm