FIGURE 1.

Thrombin abrogates leukocyte adhesion to poly(I·C)-stimulated M-SMCs. Confluent M-SMCs derived from patients with IBD were grown in DME/F-12 medium containing 10% FBS and treated without or with poly(I·C) (100 μg/ml) or TNF-α (10 ng/ml) for 18 h or thrombin (25 units/ml) for 6 h at 37 °C prior to cell adhesion assays. Adhesion of U937 cells to M-SMC cultures isolated from individual patients with IBD (P1, P2, and P3) (A) or adhesion of specific types of leukocytes (B) was performed as described under “Experimental Procedures.” (Mean data are shown with error bars representing ± S.E. between replicates (n = 3); **, p < 0.01; ***, p < 0.001.)