FIGURE 6.

Pulse-chase experiment using GFP-opsin fusion protein in ift122-deficient photoreceptor cells. A, schematic diagram of the heat-shock promoter-driven GFP-opsin expression construct used in this study. Time course of heat-shock induction of GFP-opsin in the larvae (lower panel). B–E, representative confocal images of transverse cryosections through the central retina of wild-type and ift122 mutant larvae at 4 and 24 h after heat-shock induction. The subcellular distribution of the GFP-opsin (green) was evaluated. Sections are counterstained with phalloidin (red) to visualize the outer limiting membrane and the outer plexiform layer and DAPI (blue) to visualize cell nuclei. Signal intensity in cell bodies was measured between the outer limiting membrane and the outer plexiform layer. Asterisks indicate outer segments; arrowheads indicate the outer limiting membrane, and arrows indicate the outer plexiform layer. F, graph representing signal intensities of GFP-opsin in photoreceptor cell bodies of wild-type and ift122 mutant larvae at 4 and 24 h after heat-shock induction. Percentages of GFP signal intensity in the photoreceptor cell body relative to the entire cell are shown.