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. 2016 Sep 26;291(47):24487–24503. doi: 10.1074/jbc.M115.708594

FIGURE 2.

FIGURE 2.

Depletion of HDAC1 or HDAC2 in WRN-depleted cells exacerbates the growth-suppressive effect of HU. A, population doubling levels of WRN-depleted or control (with scrambled shRNA) GM639cc1 SV40-transformed fibroblasts that were transfected with nonspecific siRNA (NS), HDAC1, or HDAC2 siRNAs (day 0) plated in triplicate into multiwell dishes (day 1) with or without treatment with 2 mm HU for 6 h on day 2 and allowed to grow for another 3 days. Cell counts in wells were averaged, and population doubling levels were determined using a formula ln(average count on dayn+1/average count on dayn)/0.693. Population doubling levels were plotted to derive linear trend lines. B and C, Western blots showing levels of HDAC1 and HDAC2 in scrambled shRNA control and WRN-depleted GM639cc1 fibroblasts transfected with nonspecific (NS) siRNA or siRNAs against HDAC1 or -2. HDAC1 or -2 levels are expressed as percentages of levels seen in cells transfected with NS siRNA. PCNA is a loading control.