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. 2016 Sep 26;291(47):24487–24503. doi: 10.1074/jbc.M115.708594

FIGURE 5.

FIGURE 5.

Co-immunoprecipitation of HDAC1, HDAC2, and WRN. A, a Western blot showing levels of WRN and HDAC1 in GM639cc1 cell nuclear extracts prior (input) and after (s/n) IP and in immunoprecipitates of an α-HDAC1 or an isotype-matching α-HA antibody. B, a Western blot showing levels of WRN and HDAC1 in nuclear extracts and α-HDAC1 immunoprecipitates from GM639cc1 and WV1 (WRN-deficient) cells. C, a Western blot showing WRN and HDAC1 levels in nuclear extracts and α-HDAC1 immunoprecipitates from control (WT) and clone #17 HDAC1 knock-out (HDAC1 KO) RD cells. D, a Western blot of WRN, HDAC1, HDAC2, and nucleolin (NCL, loading control) in whole-cell lysates of mass cultures of RD cells after CRISPR-Cas9-mediated knock-out of HDAC1 (HDAC1 KO) or HDAC2 (HDAC2 KO). WT cells express Cas9 only. Expression of HDAC1 or HDAC2 is quantified as percent of the levels seen in WT control. E, a Western blot of nuclear extracts and α-HDAC1 immunoprecipitates from control (WT), HDAC1, or HDAC2 knock-out RD cells introduced in D. F, a Western blot of nuclear extracts and α-HDAC2 immunoprecipitates from control (WT) or HDAC2 knock-out RD cells introduced in D. In E and F WRN levels in IPs are normalized to inputs and shown relative to these levels in WT controls.