ZBTB33 depletion induces differential proliferation trends in HeLa and HEK293 cells.
A and B, FACS analysis of GFP expression in HeLa and HEK293 cells 48 h after GFP mRNA transfection. C and D, immunoblot analyses of ZBTB33 protein expression in HeLa and HEK293 cells after transfection with either a scrambled (Scr) or ZBTB33 targeting siRNAs. E and F, fluorometric quantitation of cell viability after ZBTB33 depletion. G and H, FACS analysis of apoptosis in ZBTB33-depleted HeLa and HEK293 cells after 48 h. I and J, growth curves of HeLa and HEK293 cells after ZBTB33 depletion. *, p < 0.05; **, p < 0.005 by Student's t test.