FIGURE 5.

Xyl is linked to the second ribitol via a β-linkage. A, one-dimensional ¹H NMR spectrum (anomeric region) of the TMEM5 product. B, overlay of the two-dimensional ¹H-¹³C HSQC spectrum of the TMEM5 product (anomeric region) with ¹³C-decoupling during acquisition (shown in “black”) and two-dimensional ¹H-¹³C HSQC spectrum of the TMEM5 product (anomeric region) without ¹³C-decoupling (shown in “red”). The NMR spectra were collected at 298 K. The final digital resolution in the ¹H dimension was set to 4.9 Hz/point for the ¹³C-decoupled ¹H-¹³C HSQC spectrum (black) and to 1.2 Hz/point for ¹³C-coupled ¹H-¹³C HSQC spectrum (red).