TABLE 1.

¹H and ¹³C chemical shifts of TMEM5 substrate and product

Glycan	Residue	Chemical shift
		H1 C1	H2 C2	H3 C3	H4 C4	H5 C5	H6 C6
		ppm
TMEM5 substrate	Man	4.92	3.87	3.87	3.90	3.84	4.0a
		103.83	72.44	71.76	78.97	73.02	65.91
TMEM5 product	Man	4.92	3.87	3.86	3.91	3.84	4.0a
		103.81	72.49	71.75	78.94	73.06	65.83
TMEM5 substrate	GlcNAc	4.6a	3.86	3.79	3.56	3.56	3.77, 3.94
		103.9a	57.08	83.56	71.31	78.02	63.40
TMEM5 product	GlcNAc	4.6a	3.86	3.79	3.56	3.56	3.77, 3.93
		103.9a	57.09	83.57	71.31	77.99	63.45
TMEM5 substrate	GalNAc	4.6a	4.02	4.22	4.16	3.73	3.8a
		103.9a	54.15	77.44	69.51	77.36	63.62
TMEM5 product	GalNAc	4.6a	4.02	4.22	4.16	3.73	3.8a
		103.9a	54.16	77.43	69.51	77.42	63.63
TMEM5 substrate	Rbo	4.0b, 4.1b	4.0b	3.79	4.0b	4.0b, 4.1b
		69.2b	73.5b	73.82	73.5b	69.2b
TMEM5 product	Rbo	4.0a, 4.1a	4.0a	3.79	4.0a	4.0a, 4.1a
		69.2a	73.5a	73.87	73.5a	69.2a
TMEM5 substrate	Rbo′	3.65, 3.79	3.85	3.75	4.0b	4.0b, 4.1b
		64.98	74.74	74.34	73.5b	69.2b
TMEM5 product	Rbo′	3.62, 3.81	3.80	3.87	4.14	4.07, 4.17
		65.30	74.32	74.27	81.60	67.12
TMEM5 product	Xyl	4.62	3.29	3.46	3.61	3.32, 3.95
		105.62	75.91	78.26	72.03	67.84

^a Two peaks are overlapped.

^b Three peaks are overlapped.