TABLE 2.
Mass spectrometry identification for subunits of ovine complex I
| Subunit |
Mature subunit sizea |
No. of unique peptide fragments | Percent coverage | Sequence database accession no. | ||
|---|---|---|---|---|---|---|
| Bovine name | Human name | No. of amino acids | Mass | |||
| kDa | % | |||||
| 51 kDa | NDUFV1 | 445 | 48.6 | 40 | 86.6 | Uniprot no. W5PUX0 (78) |
| 24 kDa | NDUFV2 | 217 | 23.8 | 24 | 94.5 | Uniprot no. W5NRY1 (78) |
| 75 kDa | NDUFS1 | 704 | 76.9 | 65 | 92.5 | Uniprot no. W5QB34 (78) |
| 49 kDa | NDUFS2 | 430 | 49.1 | 40 | 88.1 | Uniprot no. W5PJ73 (78) |
| 30 kDa | NDUFS3 | 228 | 26.4 | 27 | 94.7 | Uniprot no. W5PB27 (78) |
| PSST | NDUFS7 | 179 | 20.1 | 14 | 87.2 | Uniprot no. W5PPP6 (78) |
| TYKY | NDUFS8 | 176 | 20.2 | 18 | 93.8 | NCB accession no. XP_011972879.1b |
| ND1 | ND1 | 318 | 35.9 | 8 | 19.2 | Uniprot no. O78747 (78) |
| ND2 | ND2 | 347 | 39.1 | 9 | 38.3 | Uniprot no. O78748 (35) |
| ND3 | ND3 | 115 | 13.1 | 3 | 23.5 | Uniprot no. O78753 (35) |
| ND4 | ND4 | 459 | 52 | 11 | 30.5 | Uniprot no. O78755 (35) |
| ND4L | ND4L | 98 | 10.8 | Uniprot no. O78754c (35) | ||
| ND5 | ND5 | 606 | 68.4 | 22 | 45.0 | Uniprot no. O78756 (35) |
| ND6 | ND6 | 175 | 19.1 | 5 | 67.4 | Uniprot no. O78757 (35) |
| 18 kDa (AQDQ) | NDUFS4 | 133 | 15.3 | 13 | 85.7 | Uniprot no. W5PE07 (78) |
| 15 kDa (PFFD) | NDUFS5 | 105 | 12.4 | 9 | 67.0 | Uniprot no. W5QFF9 (78) |
| 13 kDa | NDUFS6 | 96 | 10.6 | 10 | 86.5 | NCB accession no. XP_011980592.1b |
| 10 kDa | NDUFV3 | 75 | 8.4 | 1 | 74.7 | NCB accession no. XP_011991231.1b |
| MWFE | NDUFA1 | 70 | 8.2 | 4 | 78.9 | NCB accession no. NP_001305903.1 |
| B8 | NDUFA2 | 98 | 10.9 | 13 | 76.5 | Uniprot no. W5QAH8 (78) |
| B9 | NDUFA3 | 83 | 9.2 | 5 | 80.7 | Uniprot no. W5NYM7 (78) |
| B13 | NDUFA5 | 115 | 13.1 | 12 | 88.7 | Uniprot no. W5PNX7 (78) |
| B14 | NDUFA6 | 127 | 14.9 | 12 | 69.3 | Uniprot no. W5QC06 (78) |
| B14.5a | NDUFA7 | 112 | 12.4 | 15 | 81.2 | NCB accession no. XP_004008614.1 |
| PGIV | NDUFA8 | 171 | 20 | 19 | 90.7 | Uniprot no. W5PYA5 (78) |
| 39 kDa | NDUFA9 | 344 | 39 | 30 | 68.9 | Uniprot no. W5PI58 (78) |
| 42 kDa | NDUFA10 | 320 | 36.8 | 33 | 82.3 | Uniprot no. W5QBF5 (78) |
| B14.7 | NDUFA11 | 140 | 14.6 | 6 | 72.9 | Uniprot no. W5PAR2 (78) |
| B17.2 | NDUFA12 | 145 | 17.1 | 19 | 92.4 | Uniprot no. B9VGZ9 (79) |
| B16.6 | NDUFA13 | 143 | 16.6 | 16 | 89.5 | NCB accession no. XP_004008450.1 |
| SDAP | NDUFAB1 | 88 | 10.1 | 7 | 60.2 | Uniprot no. W5NQT7 (78) |
| MNLL | NDUFB1 | 57 | 6.9 | 6 | 70.2 | NCB accession no. XP_004018002.1 |
| AGGG | NDUFB2 | 72 | 8.5 | 4 | 36.1 | Uniprot no. W5PVD7 (78) |
| B12 | NDUFB3 | 97 | 11 | 9 | 85.7 | Uniprot no. W5Q5T4 (78) |
| B15 | NDUFB4 | 128 | 15 | 13 | 78.9 | NCB accession no. XP_004003003.1 |
| SGDH | NDUFB5 | 143 | 16.7 | 14 | 62.4 | Uniprot no. W5QHN8 (78) |
| B17 | NDUFB6 | 127 | 15.4 | 13 | 68.0 | Uniprot no. W5PZE3 (78) |
| B18 | NDUFB7 | 136 | 16.3 | 14 | 81.6 | Uniprot no. W5P5V3 (78) |
| ASHI | NDUFB8 | 158 | 18.8 | 14 | 79.1 | Uniprot no. W5Q1B0 (78) |
| B22 | NDUFB9 | 178 | 21.6 | 17 | 69.1 | Uniprot no. W5PGA3 (78) |
| PDSW | NDUFB10 | 175 | 20.8 | 18 | 75.4 | NCB accession no. XP_011999786.1 |
| ESSS | NDUFB11 | 125 | 14.4 | 14 | 74.4 | Uniprot no. W5PWF1 (78) |
| KFYI | NDUFC1 | 49 | 5.8 | 5 | 87.8 | NCB accession no. XP_004017292.1 |
| B14.5b | NDUFC2 | 120 | 14.2 | 11 | 65.6 | NCB accession no. XP_004019479.1 |
a Sites of mitochondrial targeting sequence removal were determined experimentally by peptide identification and/or by sequence alignment with the bovine subunits.
b The most reliable/best matching sequence was from O. aries musimon subspecies.