TABLE 5.

Effect of different detergents on activity of complex I from ovine mitochondrial membranes

The reaction buffer contained 0.1% of each detergent, in addition to 0.1% CHAPS and 0.25 mg/ml 4:1 DOPC/CL except where indicated.

	units/mg ± S.D.
Detergenta
No detergent, no lipids	0.478 ± 0.039
0.01% CHAPS total, no lipids	0.472 ± 0.037
0.1% CHAPS total, no lipids	0.489 ± 0.008
0.1% CHAPS total	0.527 ± 0.027
0.2% CHAPS total	0.553 ± 0.019
Digitonin	0.587 ± 0.014
DM	0.289 ± 0.010
UDM	0.273 ± 0.010
DDM	0.289 ± 0.007
TDM	0.249 ± 0.012
CYMAL-5	0.146 ± 0.019
CYMAL-6	0.137 ± 0.011
CYMAL-7	0.156 ± 0.003
ANAPOE C10E9	0.020 ± 0.003
ANAPOE C12E8	0.022 ± 0.002
Brij35	0.066 ± 0.007
DMNG	0.592 ± 0.016
LMNG	0.694 ± 0.025
GDN	0.594 ± 0.018
DHPC	0.502 ± 0.019
trans-PCCαM	0.116 ± 0.018
Flux through respiratory chain, membranes without detergent, added lipids, or DQb
Flux	0.405 ± 0.007
+ 20 μm Cyt c	0.959 ± 0.022
+ 20 μm Cyt c and 2.5 μm rotenone	0.017 ± 0.008
+ 20 μm Cyt c and 3 μm antimycin A	0.044 ± 0.005
+ 20 μm Cyt c and 400 μm KCN	0.033 ± 0.002

^a Data are for NADH/DQ activity. n = 3–6.

^b Data are for NADH/O₂ activity. n = 3–5.