TABLE 7.

DQ activity of complex I purified in the specified detergent and then exchanged into other detergents by SEC

Reaction buffer contained 0.1% CHAPS, 0.25 mg/ml 4:1 DOPC/CL, and 0.1% LMNG. Superscript RT indicates that SEC was performed at room temperature.

SEC detergent (all at 0.05%)	Activity (units/mg) ± S.D.a
DDM purification
DDM	3.20 ± 0.24
DDMRT	2.81 ± 0.07
TDMRT	3.12 ± 0.19
LMNG	3.46 ± 0.19
LMNG purification
LMNG	4.65 ± 0.12
DDM	5.26 ± 0.47
TDMRT	6.61 ± 0.43
Brij35	3.63 ± 0.23

^a n = 3–4.