TABLE 2.
Plasmids used in the study
| Plasmid | Description | Reference |
|---|---|---|
| pRK74 | A CEN-ARS plasmid (pGRB2.2) of C. glabrata carrying S. cerevisiae URA3 as a selection marker. MCS sites are flanked by S. cerevisiae PGK1 promoter at one end and by 3′ UTR of HIS3 at the other end | 60 |
| pRK942 | CgVPS15 ORF (3.4 kb) cloned in XmaI-XhoI sites in the pRK74 plasmid | 12 |
| pRK984 | CgVPS34 ORF (2.4 kb) cloned in SalI-XmaI sites in the pRK74 plasmid | 12 |
| pRK999 | A CEN-ARS plasmid (Addgene-ID 45323) of C. glabrata carrying S. cerevisiae URA3 as a selection marker. MCS sites are flanked by S. cerevisiae PDC1 promoter at one end and by 3′ UTR of HIS3 at the other end | 61 |
| pRK1018 | A CEN-ARS plasmid (Addgene-ID 45343) of C. glabrata carrying S. cerevisiae URA3 as a selection marker. MCS sites are flanked by S. cerevisiae PGK1 promoter at one end and by 3′ UTR of HIS3 at the other end. yEGFP is introduced at the C terminus | 61 |
| pRK1037 | CgVPS34N750K (2.4 kb) cloned in SalI-XmaI sites in the pRK74 plasmid | 12 |
| pRK1056 | CgMRS4 ORF (0.92 kb) cloned in XmaI-XhoI sites in the pRK999 plasmid | Lab collection |
| pRK1058 | CgCCC1 ORF (0.92 kb) cloned in BamHI-SalI sites in the pRK999 plasmid | Lab collection |
| pRK1100 | CgFTR1 ORF (1.22 kb) cloned in XbaI-XmaI sites in the pRK1018 plasmid | This study |
| pRK1102 | CgFET3 ORF (1.90 kb) cloned in XbaI-XmaI sites in the pRK1018 plasmid | This study |