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. 2016 Oct 10;291(47):24747–24755. doi: 10.1074/jbc.M116.749408

FIGURE 2.

FIGURE 2.

miR-30a inhibits Ubc9 expression in human white adipocytes. A–E, the gene expression levels of published miR-30a targets were analyzed in human adipocytes transfected with control or miR-30a mimics for 48 h. mRNA levels of Ubc9 (A), ATG5 (B), RIP140 (C), p53 (D), and BECN1 (E) were analyzed by qPCR. rel, relative. F, immunoblotting verified exclusive suppression of Ubc9 expression using samples analyzed by qPCR. *, p < 0.05 relative to cells transfected with control mimic (n ≥ 3 independent experiments). G, TargetScan cumulative weighted context++ scores of published miR-30a targets suggest that the miR-30/Ubc9 interaction is favorable relative to other targets. More negative values indicate a stronger interaction potential. nc, not-called.