FIGURE 6.

Recombinant KDM1A is inhibited by hydrogen peroxide. A, exogenously applied H₂O₂ inhibits recombinant KDM1A with an apparent IC₅₀ value of 630 nm, error bars indicate S.D. B, inhibition of recombinant KDM1A by H₂O₂ or dimedone plus H₂O₂ is reversible by the addition of DTT. KDM1A was pre-incubated with dimedone (100 μm) or vehicle for 10 min, then treated with H₂O₂ (100 μm or 250 μm) for 10 min prior to reduction. Error bars indicate S.D.; ***, p < 0.001 by 2-tailed t test with correction for multiple comparisons. For both A and B, demethylation of H3K4me2 starting material was detected by LC-MS after 1-h reaction.