TABLE 1.
Hits identified through RIT-seq screening
Hit list showing RPKM for barcoded reads (×103); only genes with RPKM >1000 for barcoded reads and RPKM >200 for all mapped reads are included. MTX, methotrexate screen; RTX, raltitrexed screen.
| Gene ID | Gene description | MTX reads | RTX reads |
|---|---|---|---|
| Tb927.8.3620 | Folate transporter, putative | 387.4a | 425.6a |
| Tb927.8.3650 | Folate transporter, putative | 190.1a | 139.4a |
| Tb927.8.3630 | Folate transporter, putative | 107.0a | 125.5a |
| Tb11.v5.0766 | Folate transporter, putative (truncated) | 85.4a | 78.9a |
| Tb927.11.14360 | Mitochondrial carrier protein (MCP2) | 16.6a | 4.0a |
| Tb927.7.1600 | C-1-tetrahydrofolate synthase, cytoplasmic, putative | 0 | 57.3a |
| Tb927.11.12460 | Hypothetical protein, conserved | 0 | 3.6 |
| Tb927.8.1040 | Protein phosphatase inhibitor, putative | 2.1 | 0.5 |
| Tb927.9.5960 | Succinate dehydrogenase, putative | 1.7 | 0 |
| Tb927.10.7520 | Folylpolyglutamate synthase, putative (FPGS) | 0 | 1.2 |
| Tb927.8.5840 | Hypothetical protein, conserved | 1.2 | 0 |
a Primary hit with >1 fragment per gene; others, secondary hits with only one fragment per gene. GeneID and description information is from tritrypdb.org.