TABLE 2.
Effect of RNAi knockdown of folate transporters FT1–3 on susceptibility to antifolates
EC50 values (nm) of drugs against uninduced and induced FT knockdown cells. Results are weighted means ± weighted error of three independent experiments.
| Drug | 3 days |
6 days |
||||
|---|---|---|---|---|---|---|
| Minus tetracycline | Plus tetracycline | -Fold shift | Minus tetracycline | Plus tetracycline | -Fold shift | |
| Methotrexate | 14.5 ± 0.4 | 444 ± 16.9 | 30.6 | 8.73 ± 0.39 | 321 ± 12.8 | 36.8 |
| Raltitrexed | 11.2 ± 0.3 | 368 ± 13.7 | 32.9 | 5.83 ± 0.20 | 227 ± 9.68 | 38.9 |
| Pyrimethamine | 2,500 ± 120 | 200 ± 11.0 | 0.08 | 20,40 ± 165 | 142 ± 10.4 | 0.07 |
| Nolatrexed | 36,200 ± 1,910 | 16,700 ± 957 | 0.46 | 39,900 ± 2,480 | 15,600 ± 886 | 0.39 |
| Melarsoprol | 1.73 ± 0.09 | 1.70 ± 0.08 | 0.98 | 1.26 ± 0.60 | 1.29 ± 0.59 | 1.02 |