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. 2016 Nov 15;194(10):1208–1218. doi: 10.1164/rccm.201601-0002OC

Figure 2.

Figure 2.

(A) Cytometric gating strategy. Lung macrophages were identified as positive for CD45-PE-CF594, HLA-DR-APC-Cy7, and Lin1-FITC. (B) Specific mean fluorescence intensity (SMFI) of surface major histocompatibility complex–related protein 1 (MR1), HLA-DR, and HLA-ABC on macrophages from resected human lung either uninfected or infected with live nontypeable Haemophilus influenzae (NTHi) at multiplicity of infection (MOI) 1 and MOI 10 (n = 6 or 4). (C) SMFI of surface MR1, HLA-DR, and HLA-ABC on monocytes differentiated into macrophages from peripheral blood of healthy volunteers either uninfected or infected with live NTHi at MOI 1 or with paraformaldehyde (PFA)-NTHi at MOI 1 (n = 9, 10, and 7, respectively). Bars indicate medians. P values represent Wilcoxon signed-rank tests. *P < 0.05; **P < 0.01. APC = allophycocyanin; Cy7 = cyanine 7; DR = D related; FITC = fluorescein isothiocyanate; HLA = human leukocyte antigen; Lin1 = lineage 1; PE = phycoerythrin.