Figure 3.
Specific mean fluorescence intensity (SMFI) of major histocompatibility complex–related protein 1 (MR1) on monocyte-derived macrophages (MDMs) from peripheral blood either not treated or treated with (A) TNF-α, IL-6, or IFN-γ (10 ng/ml) (n = 6) or (B) LPS (100 ng/ml) (n = 6) or (C) infected with influenza virus X31 (n = 3). (D) MR1 mRNA from MDMs either uninfected or infected with live nontypeable Haemophilus influenzae (NTHi) at multiplicity of infection (MOI) 1 or with paraformaldehyde (PFA)-fixed NTHi at MOI 1. RNA expression is expressed as ΔΔCt normalized to β2M. Bars indicate medians. TNF = tumor necrosis factor.