Fig. 1.

VSMC contractile protein expression is increased by G6PD inhibition and deficiency. Isolated coronary arteries were treated with G6PD inhibitor 6-aminonicotinamide (6AN; 1 mM; n = 5) for 12 h. A: expression of contractile proteins SM22α (TAGLN), calponin (CNN1) and smooth muscle myosin heavy chain (MYH11) were measured by immunoblotting. Proteins were normalized to glyceraldehyde-6-phosphate dehydrogenase (GAPDH). B: Volcano plot demonstrates several genes are up- and downregulated in aorta of G6PD-deficient mice (n = 3). C: a representative immunoblot of MYH11 expression in G6PD-deficient mice aorta is shown (n = 3). Green circles: significant (P < 0.05); yellow circles: significant, but unreliable replicate data; red circles: significant (P < 0.05), but inadequate Log2_FoldChange; orange circles: not significant but considerable Log2_FoldChange; gray Circles: not significant.