Table 5.
Variables from hepatitis A virus Ag-stimulated peripheral blood mononuclear cell phenotypes from patients with acute hepatitis A infection and healthy subjects
| Phenotypes/cytokines (HAVAg) | HC (n = 10) | AH (n = 8) | ALF (n = 8) | HC vs AH | HC vs ALF | AH vs ALF |
| PI of CD3+ | 1.09 ± 0.85 | 3.15 ± 1.92 | 3.34 ± 2.29 | 0.0053 | 0.0044 | ns |
| (0.64-1.55) | (1.54-4.76) | (1.42-5.25) | ||||
| CD4+CD25+FoxP3+ (%) | 0.85 ± 0.96 | 1.0 ± 0.97 | 3.19 ± 1.49 | ns | 0.0011 | 0.0070 |
| (0.34-1.37) | (0.18-1.81) | (1.95-4.44) | ||||
| CD4+CD29+CD44+ (%) | 11.99 ± 6.43 | 27.93 ± 8.16 | 5.46 ± 5.92 | 0.0008 | 0.0077 | 0.0006 |
| (8.55-15.42) | (21.1-34.76) | (0.50-10.42) | ||||
| CD8+CD29+CD44+ (%) | 12.65 ± 4.31 | 30.13 ± 6.74 | 36.05 ± 10.59 | 0.0001 | 0.0001 | ns |
| (10.35-14.95) | (24.49-35.77) | (27.19-44.90) | ||||
| CD3-CD56+CD16- (%) | 0.19 ± 0.20 | 0.30 ± 0.19 | 1.33 ± 0.85 | ns | 0.0009 | 0.0005 |
| (0.05-0.34) | (0.13-0.46) | (0.62-2.04) | ||||
| CD3-CD56lowCD16+ (%) | 4.28 ± 2.22 | 10.09 ± 8.94 | 14.24 ± 11.81 | ns | ns | ns |
| (2.70-5.87) | (2.61-17.5) | (4.36-24.12) | ||||
| CD3+CD56+CD16+ (%) | 1.67 ± 2.71 | 4.25 ± 4.06 | 15.06 ± 7.74 | 0.0110 | 0.0003 | 0.0019 |
| (0.22-3.12) | (0.85-7.65) | (8.58-21.53) | ||||
| IL-6 (pg/mL) | 50.49 ± 76.14 | 76.41 ± 93.18 | 139.7 ± 165.9 | ns | ns | ns |
| (9.92-91.06) | (-1.46-154.3) | (0.98-278.4) | ||||
| TNFα (pg/mL) | 92.49 ± 133.4 | 23.96 ± 28.92 | 1.63 ± 1.01 | ns | 0.0089 | 0.0098 |
| (21.42-163.6) | (-0.21-48.14) | (0.78-2.48) | ||||
| IL-10 (pg/mL) | 10.39 ± 13.97 | 52.78 ± 62.06 | 164.3 ± 75.56 | 0.0297 | 0.0001 | 0.0148 |
| (2.94-17.84) | (0.89-104.7) | (101.1-227.5) | ||||
| IFNγ (pg/mL) | 0.88 ± 1.08 | 106.6 ± 183.9 | 1095 ± 1962 | 0.0035 | 0.0001 | 0.0499 |
| (0.30-1.46) | (-47.14-260.4) | (-546-2735) | ||||
| IL-8 (pg/mL) | 88.64 ± 45.40 | 148.9 ± 54.77 | 150.2 ± 72.19 | 0.0131 | 0.0110 | ns |
| (64.44-112.8) | (103.1-194.7) | (89.84-210.5) | ||||
| IL-17 (pg/mL) | 3.36 ± 3.75 | 32.61 ± 38.30 | 7.58 ± 5.43 | 0.0008 | ns | 0.0499 |
| (1.36-5.36) | (0.59-64.64) | (3.05-12.13) |
The differences between the hepatitis A-induced acute liver failure (ALF) patients, the self-limited acute hepatitis A (AH) patients and the healthy control (HC) subjects were evaluated by intergroup comparisons using the Mann-Whitney U-test. The significance cutoff for all statistical analyses was defined as P < 0.05. IL: Interleukin; IFNγ: Interferon gamma; TNFα: Tumor necrosis factor alpha; ns: Not significant; PI: Proliferation index.