Figure 5. Atg1 and Atg8a promote selective removal of mtDNA^Δ.

All data presented are derived from Supplementary Table 3. (a) Relative abundance of different mtDNA species determined using qPCR and tpRCA. Bars depict mean±1 s.d. Supplementary Table 3 shows numbers of biological replicates. (b) Images from flight muscle of the indicated genotypes, hybridized for tpRCA as in Fig. 3. Scale bar, 10 μM. Statistical significance is estimated as compared with levels of the corresponding species in mitoAflIII flies using Student's t-test with unequal variance. ^NSP>0.05, **P<0.01 and ***P<0.001. Note that number of nucleoids labelled in each sample in b is not reflective of total muscle mtDNA levels, which are estimated in a using qPCR and comparisons with nuclear DNA (mtDNA^total/nucDNA). NS, not significant.