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. 2016 Nov 18;6:37238. doi: 10.1038/srep37238

Table 1. Timeline of the experiment.

Day Who Event Observation Place Sample size
C V1+ V2+
PND-30 to PND-5 Sows Positive and negative handling treatments   Rearing pen 10 10 10
D0 Sows Delivery   Rearing pen 9 10 9
D2 Piglets Social Isolation Test 1 (SIT1) (5 min) Simultaneous broadcast of V1 and V2 voices Testing pen 1 × 2 m 35 36 39
D7 Piglets Social Isolation Test 2 (SIT2) (10 min) Piglet alone during two consecutive phases of 5 minutes each: Phase 1: No sound broadcast Phase 2: Sound broadcast (either V1 or V2) Testing pen 2 × 2 m 34 (V1: 17, V2: 17) 36 (V1: 18, V2: 18) 37 (V1: 19, V2: 18)
D14 Piglets Social Isolation Test 3 (SIT3) (5 min) Simultaneous broadcast of V3 and V4 voices Testing pen 1 × 2 m 20 20 20
D21 Piglets Social Isolation Test 4 (SIT4) (5 min) Piglet with a motionless human and broadcast of the voice associated prenatally with the maternal positive treatment (i.e. V1 for V1+ and V2 for V2+) Testing pen 2 × 2 m 16 18 18