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. 2016 Sep 9;15(6):1082–1091. doi: 10.1111/acel.12512

Figure 3.

Figure 3

BDNF protein and gene expression in response to exercise and retinal injury. Levels of total BDNF protein (A) and BDNF mRNA (B) were measured in soleus muscle and retina of 12‐month‐old C57BL/6J mice that were exercised daily by swimming for 7 consecutive days. (C) Retinal protein expression of BDNF was also assessed by immunohistochemistry. Representative images show BDNF labelling (red) in the RGC layer of retinal cryosections. Scale bar = 50 μm. Injured eyes from nonexercised and exercised 12‐month‐old C57BL/6J mice were also assessed for BDNF protein (C,D) and gene (E) expression. Data are expressed relative to control (A,B) or exercise (D,E) values; mean ± SEM; n = 10; *P < 0.05 by Student's t‐test.