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. 2016 Nov 18;6:36698. doi: 10.1038/srep36698

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Copyright © 2016, The Author(s)

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(a) Representative micrographs showing immunofluorescent staining of kidney specimens procured on day 7 for F4/80 and mannose receptor (MR). Sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bar = 50 μm. White arrows indicate the dually positive cells in the sections. (b) Flow cytometry analysis of isolated single kidney cells for cells positive for MR and F4/80; cells were gated on F4/80⁺ macrophages. (c) The percentage of F4/80⁺MR⁺ cells among all F4/80⁺ cells as determined by flow cytometry. ^*P < 0.001 vs FA; ^#P < 0.001 vs FA+TS(E); (n = 9). (d) Representative immunoblots of Arginase I, MR, and F4/80 in cortical kidney lysates prepared on day 7. (e) and (f) Relative abundance of Arginase I (e) and MR (f) in immunoblots expressed as densitometric ratios of Arginase I/F4/80 or MR/F4/80. ^*P < 0.001 vs FA; ^$P = 0.041 vs FA+TS(E); ^@P = 0.002 vs FA; ^△P = 0.029 vs FA+TS(E); (n = 9).