Figure 2. Protein interaction network of Oxa-23 in AB5075.

(a) The network included 18 Oxa-23 direct interaction partners (6 membrane, 4 uncharacterized and 8 soluble proteins) and four extended interaction partners (Omp33, AdeK, AdeC and ABUW_0724). Node number indicates cross-linked lysine residues. Nodes were grouped by proteins. Node colour shows the primary subcellular localization of the proteins. Blue, membrane proteins; green, uncharacterized proteins; light red, soluble proteins; dark red, soluble proteins predicted to localize in periplasm. Edge colours illustrate the specific types of Oxa-23 cross-links. Blue edges are intra-protein cross-links of Oxa-23. Orange or red edges are inter-protein cross-links of Oxa-23 to OmpA and YiaD, or to other interactors. Grey edges are cross-links formed between Oxa-23 interactors. The network was generated with Cytoscape 3.0.2 (ref. ⁷³). (b) Inter-protein cross-links of OmpA-Oxa23 and YiaD-Oxa23 annotated with protein primary sequences and protein domains. Homodimeric cross-linked sites of Oxa-23 K102, K104, K107 and K194 are highlighted in blue. Cross-links are colored based on the cross-linked lysine residues of OmpA and YiaD.