Table 2. MICs for Δoxa23 complementation mutants.
| Strains | Meropenem MIC (μg ml−1) | Imipenem MIC (μg ml−1) |
|---|---|---|
| AB5075 WT | 8 | 8 |
| Δoxa23 | 1 | 0.5 |
| Δoxa23+pMMB.A1-oxa23-WT | 8 | 8 |
| Δoxa23+pMMB.A1-oxa23-K82A | 1 | 0.5 |
| Δoxa23+pMMB.A1-oxa23-DKK60AAA | 8 | 6–8 |
| Δoxa23+pMMB.A1-oxa23-D238A | 6–8 | 6 |
| Δoxa23+pMMB.A1-oxa23-DKK60AAA/D238A | 3–4 | 2 |
MIC, minimal inhibitory concentration; WT, wild type.
Δoxa23 deletion mutant was complemented with WT oxa-23 gene or oxa-23 site-directed mutants using pMMB.A1 vector. The MIC (μg ml−1) is the lowest antibiotic concentration preventing the lawn growth of the bacteria. Range values indicate variations of MIC values detected with individual isolates of each strain. Four individual isolates were examined for each strain. See Supplementary Figs 12 and 13 for more details.