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. 2016 Nov 18;6:37421. doi: 10.1038/srep37421

Figure 7. The effect of PTPN9 on cell cycle progression in breast cancer cells.

Figure 7

(A–D) Cell cycle profiles were analyzed using flow cytometry after the transfection of MCF-7 cells with equal doses of siRNA-NC or PTPN9 siRNA, or with equal doses of plasmid-NC or PTPN9 overexpression plasmid. The untransfected cells (Mock) serve as the negative control. The panel shows histograms of cell numbers (y axis) against DNA content (x axis) determined by measuring fluorescence intensity. Numbers denote the percentages of cells in the G1/G0, S and G2/M phases. (A and B) Representative images; (C and D) quantitative analysis. (E and F) Quantitative RT-PCR analysis of the relative expression levels of Cyclin D1, CDK6, CDK4 and p21 mRNA in MCF-7 cells transfected with equal doses of siRNA-NC or PTPN9 siRNA, or with equal doses of plasmid-NC or PTPN9 overexpression plasmid. The untransfected cells (Mock) serve as the negative control. (G) Western blot analysis of Cyclin D1, CDK6, CDK4 and p21 protein levels in MCF-7 cells transfected with equal doses of siRNA-NC or PTPN9 siRNA, or with equal doses of plasmid-NC or PTPN9 overexpression plasmid. The untransfected cells (Mock) serve as the negative control. *P < 0.05; **P < 0.01; ***P < 0.001.