Table 1.
Alternative topology tests
| Alternative topologies | P-value | |
|---|---|---|
| Consensus | Unconstrained 47 protein phylogeny | 1.000 |
| Topology 1 | D. polycarpum is outgroup to branch I | 1e-04 |
| Topology 2 | D. polycarpum is outgroup to group 1 | 1e-04 |
| Topology 3 | D. polycarpum is outgroup to branch II | 6e-74 |
| Topology 4 | Root between group 1 and group 2, as in SSU rDNA tree | 5e-74 |
| Topology 5 | D. polycephalum is outgroup to branch I | 3e-68 |
| Topology 6 | P. violaceum is outgroup to group 3 | 2e-43 |
| Topology 7 | P. violaceum groups with P. pallidum | 4e-29 |
| Topology 8 | A. ellipticum groups with other Acytostelids | 5e-47 |
| Topology 9 | A. ellipticum groups with Polysphondylids, as in SSU rDNA tree | 5e-40 |
The probabilities (p-value) of the alternative tree topologies listed in Fig. 5 were investigated using the approximately unbiased (AU) test in CONSEL v0.20 [25]. Trees with constrained nodes were generated using RaxML and log likelihood values for the consensus tree and alternative trees were used as input for the AU test