Fig. 3.

CD274 maintains the Cyclin D2 level to accelerate AML development. a, b GO and KEGG analyses of differentially expressed genes in WT and CD274-null LICs from RNA sequencing data are shown. Candidate genes involved in the biologic progress and the pathway are highlighted. c Candidate genes were further confirmed with WT and CD274-null LICs using real-time RT-PCR. d Cyclin D2 levels in WT and CD274-null LICs were detected by Western blotting analysis. e Long-rank test analysis for the overall survival of the recipient mice receiving WT AML cells, CD274-null AML cells, and Cyclin D2-overexpressed CD274-null AML cells (n = 4–5). f Cyclin D2 level was examined in WT AML cells, CD274-null AML cells, and Cyclin D2-over-expressed CD274-null AML cells in e. g The stages of cell cycle were analyzed by Pyronin Y and Hoechst 33342 staining in WT LICs, CD274-null LICs, and Cyclin D2-over-expressed CD274-null LICs