Fig. 2.

Expression pattern of Par3 in JHOC5 ovarian cancer cells. a Par3 protein level in seven ovarian cancer cell lines (JHOC5, JHOC8, OVISE, OVTOKO, TOV21, RMG1, and SKOV3). Semiconfluent cells were harvested for western blotting. Cell lysates were resolved by SDS-PAGE and immunoblotted with an antibody against Par3 or E-Cadherin. Blots are representative of at least three experiments. b Subcellular fractionation assay. JHOC5 cells were fractioned into cytosol (F1), membrane (F2), nucleus (F3), and cytoskeleton (F4) pools and Par3 was detected by western blotting. Loading controls used were α-Tubulin, TFR, p84, and vimentin for the cytosol, membrane, nucleus, and cytoskeleton fractions, respectively. c Immunofluorescence analysis of Par3 expression in JHOC5 cells and control HaCaT cells. Cells were grown on coverslips and then fixed and stained with the anti-Par3 antibody and DAPI. Scale bar indicates 10 μm