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. 2016 Oct 31;197(11):4219–4227. doi: 10.4049/jimmunol.1601094

FIGURE 4.

FIGURE 4.

Lung APCs produce IL-27 in response to R848, which impacts Th2/Th1 cytokine production and tolerogenic marker expression in murine and human APCs. Gating strategy for AMs and DCs in murine lungs (A) and staining strategy used for identification of human monocytes and DCs in peripheral blood (B) are shown. Murine AMs and DCs sorted from lungs of wild-type mice as well as human DCs and monocytes display increased expression of PD-L1 on stimulation with R848 (C). Additionally, murine AMs and DCs produce IL-27 on exposure to R848 (D). The dose-dependent influence of in vitro stimulation with recombinant murine IL-27 (rmIL-27) on IFN-γ production by murine DCs and AMs (E) and expression of PD-L1 (F) are shown. Reduced Th2 cytokine and IFN-γ production by BLN cells of allergic mice restimulated in presence of rmIL-27 (G). Enhanced expression of PD-L1 on human DCs and monocytes after in vitro stimulation with recombinant human IL-27 (rhIL-27) (H) is shown. Flow cytometry histograms are representative of one of two murine experiments or of DCs and monocytes from five human donors. Graphs display a summary of two to three independent experiments with means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. rhIL-27, recombinant human IL-27; rmIL-27, recombinant murine IL-27.