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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Sep 4;1861(11):1808–1815. doi: 10.1016/j.bbalip.2016.09.001

Figure 4.

Figure 4

Changes in phosphorylation of key proteins in serum-starved MCF-7 cells show that treatment with D-3-deoxy diC8PI leads to activation of the p38 MAPK pathway. (A) Incubation of MCF-7 cells with 100 μM D-3-deoxy-diC8PI (D-3-deoxy) or 16 μM perifosine in the absence of serum for 3..5 h led to phosphorylation of p38MAPK on Thr180/Thr182, pMAPKAPK-2 on Thr222 and hyperphosphorylation of pCREB on Ser133. (B) Cells were treated with DMSO or 10 μM SB203580, a MAPK inhibitor, for 1 h prior to incubation with 100 μM of D-3-deoxy-diC8PI (3.5 h for pCREB and 6.5 h for PARP). The presence of SB203580 eliminates CREB hyperphosphorylation but not PARP cleavage induced by D-3-deoxy-diC8PI. (C) Cells were treated with DMSO (solid grey) or 10 μM SB203580 (hatched) in serum free media for 6 h prior to 24 h incubation with various concentrations of D-3-deoxy-diC8PI.