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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Sep 4;1861(11):1808–1815. doi: 10.1016/j.bbalip.2016.09.001

Figure 5.

Figure 5

(A) Regulation of levels of cyclins D1 and D3 by D-3-deoxy-diC8PI. Cells were pretreated with DMSO (lanes 1 and 2) or 10 μM SB203580 (lanes 3 and 4) for 1 h prior to incubation with PBS (lanes 1 and 3) or 100 μM D-3-deoxy-diC8PI for 3.5 h. Detergent whole cell extracts were prepared and analyzed by western blot analysis for cyclin D1 and D3 and phosphorylation of pRb on Ser780 and Ser807/811. The blot was stripped and probed for constitutive expression of HSP90. (B) D-3-deoxy diC8PI inhibits DNA synthesis.