Fig. 1.

PI3K activity is required for HCV replication. (a) SGR-Feo-JFH-1-harbouring cells were mock treated (lanes 1, 4, 7) or treated with either daclatasvir (DCV) (100 pM) (lanes 2, 5, 8) or wortmannin (Wort; 2 μM) (lanes 3, 6, 9) for 24 h, 48 h and 72 h, respectively, prior to lysis for analysis by Western blot with the antibodies indicated. (b) Densitometry analysis of the Western blot expression data in (a). The ratios of NS5A : GADPH (black bars) or LC3-II : LC3-I (grey bars) are shown. *P < 0.05. (c). Luciferase activity is presented as values normalized to the mock-treated cells at each time point. Data represent the mean ± se (n = 3).