Fig. 8.

Partial colocalization of NS4B or NS5A expressed alone or in the context of the remaining replicase proteins with DFCP1. (a) SGR-Feo-JFH1-harbouring cells were fixed and stained with antibodies to NS5A and NS3. Huh7 cells were co-transfected with expression plasmids for mCherry–DFCP1 and (b) JFH-1 NS3-5B, (c) NS5A or (d) NS4B–GFP for 48 h. In (b) and (c) cells were fixed and stained with a sheep polyclonal antiserum to NS5A (green). Bars, 10 μm. (e) Colocalization analysis of images from (a–d). Mean Manders’ overlap coefficients were derived from >10 cells for each condition. Data represent the mean ± sd. *P < 0.05. **P < 0.005.