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. 2016 Sep;7(9-10):309–322. doi: 10.18632/genesandcancer.122

Figure 4. CETP contributes to cancer cell growth and survival and is upregulated in breast cancer patients.

Figure 4

(A) CETP gene silencing by siRNA and western blot analysis of CETP protein expression in CETP siRNA cells. (B) Effect of CETP mRNA silencing on growth of MCF-7 cells as measured by cell counting over a period of six days. (C) Comparison of cholesterol levels in the MCF-7 control (ctrl) siRNA and CETP knockout cells. Data shown are the mean ± SD of quadruplicate wells/condition in three independent experiments (n = 3) (P ≤ 0.05, t-test). (D) The effect of 10 μM PL or AP treatment on MOMP; (E) Capase-3/7 activity (10, 25 and 50 μM Tamoxifen was used as a positive control); and (F) apoptosis in MCF-7 ctrl siRNA and CETP siRNA knockout cells. Data shown are representative of the mean ± SD of quadruplicate wells/condition in at least two independent experiments (n=2) (*P ≤ 0.05, t-test).