Figure 2. Uptake of transferrin (Tfn) and epidermal growth factor (EGF) in micropatterned cells. (A) Maximum intensity projection (MIP) of fluorescently marked Tfn in single crossbow-shaped micropatterned cells at 1 min, 5 min, 10 min and 30 min after ligand addition (upper panel) and corresponding 3D density maps of the 50% probability contour for n cells (lower panel). Density maps represent the smallest regions in which 50% of fluorescent structures are found. (B) MIP of fluorescently marked EGF in single crossbow-shaped micropatterned cells at 1 min, 5 min, 10 min and 30 min after ligand addition (upper panel) and corresponding 3D density maps of the 50% probability contour for n cells (lower panel). Density maps represent the smallest regions in which 50% of fluorescent structures are found. (C) Merged 3D density maps of fluorescently marked Tfn (green) and EGF (red) at different time points. For ease of visualization, the height (Z axis) has been stretched 5-fold.