Fig.6.
Effect of amyloid-beta peptides on synaptophysin expression in the absence or the presence of HSA. Neurons in primary (3 DIV) culture were incubated for 20 h in DMEM medium with three different Aβ peptides: Aβ25-35, Aβ40, or Aβ42 (30 μM) in the absence or the presence of HSA (30 μM) or as HSA-Aβ complexes (30 μM). Then proteins were obtained and analyzed by western blot. Results are expressed as percentages compared to non-treated cells and are means±SEM (n≥7). One-way ANOVA and Dunnett Test were applied in order to compare different treatments vs. control. Distinct characters are used to indicate statistically different groups as compared to the control (p < 0.05). See Supplementary Figure 2 for raw western blot.