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. Author manuscript; available in PMC: 2017 Jul 30.
Published in final edited form as: Int Rev Cell Mol Biol. 2016 Jul 30;327:195–261. doi: 10.1016/bs.ircmb.2016.06.004

Figure 4. Centrosome translocation and docking at the IS.

Figure 4

(A) Contact between the APC and T cell triggers a rapid translocation of the MTOC toward the IS (2–5 min). Migrating lymphocytes show a polarized shape. Cognate contact with a specific APC promotes clustering of TCR/CD3 complexes and production of local DAG. PKCε and η cluster to DAG and help actin polymerization. PKCθ clusters to actin cytoskeleton and regulates its dynamics. Dynein/dynactin complexes and Myosin IIA help the coalescence of TCR/CD3 microcluster and MTOC translocation to the IS in formation. (B) Activation of TCR/CD3 through the phosphorylation of the ITAMs by Lck and Fyn members of src family of kinases promotes MTOC translocation to the IS. This movement depends on the dynein/ dynactin complex and also requires the interaction of ADAP with the integrins to generate the pulling force toward the IS.