Fig. 3.

EOMA CM contains factors that are sensitive to heat treatment and lipid depletion. (A) MF survival in DMEM and EOMA CM heat treated at 56 °C for 1 or 4 h. All groups were significantly different from one another, except for DMEM vs. DMEM HT 56 °C. Median survival was calculated as 2.88 days for DMEM HT 56 °C, 3.6 days for DMEM, 7.69 days for EOMA CM HT 56 °C 4 h, 8.82 days for EOMA CM HT 56 °C, and 16.57 days for EOMA CM. (B) MF survival in DMEM and EOMA CM heat treated at 100 °C for 1 h. DMEM HT 100 °C + FBS = DMEM heat treated at 100 °C for 1 h followed by subsequent addition of 10% FBS. EOMA CM HT 100 °C + FBS = EOMA CM heat treated at 100 °C for 1 h followed by subsequent addition of 10% FBS. Groups were statistically different from one another except for: DMEM vs. EOMA CM HT 100 °C + FBS, DMEM HT 100 °C vs. DMEM HT 100 °C + FBS, DMEM HT 100 °C vs. EOMA CM HT 100 °C, and DMEM HT 100 °C + FBS vs. EOMA CM HT 100 °C. Median survival was calculated as 1.34 days for EOMA CM HT 100 °C, 1.53 days for DMEM HT 100 °C, 1.54 days for DMEM HT 100 °C + FBS, 2.49 days for DMEM, 2.55 days for EOMA CM 100 °C, and 16.31 days for EOMA CM. (C) MF survival in DMEM and EOMA CM depleted of lipids and lipoproteins with LRA. DMEM LRA = DMEM treated with LRA. DMEM LRA + 10% FBS = DMEM treated with LRA, followed by subsequent addition of 10% FBS. EOMA LRA = EOMA CM treated with LRA. EOMA LRA + 10% FBS = EOMA CM treated with LRA, followed by subsequent addition of 10% FBS. EOMA CM was statistically different from all other groups. Additionally, DMEM vs. DMEM LRA, DMEM LRA vs. EOMA CM LRA, and DMEM LRA vs. EOMA CM LRA + FBS were statistically different from one another. Median survival was calculated as 1.90 days for DMEM LRA, 1.99 days for DMEM LRA + FBS, 2.36 days for EOMA CM LRA + FBS, 2.62 days for EOMA CM LRA, 2.88 days for DMEM, and 16.03 days for EOMA CM.