Fig. 5.

C3 is upregulated in EOMA CM and modestly prolongs MF survival. (A) Comparative 2D DIGE gel of EOMA CM (green) and RBL-2H3 CM (red). 32 spots that exhibited >1.5 fold upregulation in EOMA CM were analyzed by mass spectrometry (Table 1). A cluster analysis was performed to determine which functional groups were enhanced in EOMA CM (Table 2). (B) Supplementation of DMEM with proteins from Table 2 that were categorized as secreted/extracellular. A single dose of 20 μg of recombinant mouse C3, recombinant apolipoprotein E, or native albumin was added to DMEM with 10% FBS. DMEM + Recombinant C3 was statistically different from all other groups. Median survival was calculated as 2.15 days for DMEM + Recombinant ApoE, 2.41 days for DMEM + Native Albumin, 2.88 days for DMEM, and 3.89 days for DMEM + Recombinant C3.