Table 1.
Mass spectrometry identification of 32 spots exhibiting >1.5 fold upregulation in EOMA CM compared to RBL-2H3 CM.
| Spot number | Match confidence | Protein name [species] | Accession no. | Protein MW | Protein pI |
|---|---|---|---|---|---|
| 1 | High | Serum albumin | ALBU_MOUSE | 68,648 | 5.8 |
| 11 | Low | Alstrom syndrome protein 1 homolog | ALMS1_MOUSE | 359,993 | 6.0 |
| 15 | High | Serum albumin | ALBU_MOUSE | 68,648 | 5.8 |
| 18 | High | Voltage-dependent R-type calcium channel subunit alpha-1E | CAC1E_MOUSE | 257,071 | 8.5 |
| 25 | High | Nucleolar RNA helicase 2 | DDX21_MOUSE | 93,493 | 9.2 |
| 28 | High | Serum albumin | ALBU_MOUSE | 68,648 | 5.8 |
| 30 | High | Complement C4 [Bos taurus] | gi|31563307 | 101,820 | 6.2 |
| 36 | High | Complement C3 | CO3_MOUSE | 186,366 | 6.3 |
| 41 | High | Keratin, type II cytoskeletal 6B | K2C6B_MOUSE | 60,285 | 8.5 |
| 42 | High | Ectodysplasin-A receptor-associated adapter protein | EDAD_MOUSE | 23,738 | 5.0 |
| 43 | High | Ectodysplasin-A receptor-associated adapter protein | EDAD_MOUSE | 23,738 | 5.0 |
| 44 | High | Apolipoprotein E | APOE_MOUSE | 35,844 | 5.6 |
| 46 | High | Collagen alpha-1(I) chain | CO1A1_MOUSE | 137,948 | 5.7 |
| 47 | High | Inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein) [Bos taurus] | gi|59857769 | 101,446 | 6.3 |
| 48 | High | Serum albumin | ALBU_MOUSE | 68,648 | 5.8 |
| 49 | High | Cathepsin L1 | CATL1_MOUSE | 37,523 | 6.4 |
| 52 | Low | Transcription factor EC | TFEC_MOUSE | 35,122 | 6.3 |
| 53 | Low | Very long-chain specific acyl-CoA dehydrogenase, mitochondrial | ACADV_MOUSE | 70,831 | 8.9 |
| 54 | High | Serum albumin | ALBU_MOUSE | 68,648 | 5.8 |
| 55 | High | Complement C3 | CO3_MOUSE | 186,366 | 6.3 |
| 56 | High | Complement C3 | CO3_MOUSE | 186,366 | 6.3 |
| 58 | High | Serum albumin | ALBU_MOUSE | 68,648 | 5.8 |
| 61 | High | Proteasome subunit alpha type-7 | PSA7_MOUSE | 27,838 | 8.6 |
| 65 | High | Lymphoid-restricted membrane protein | LRMP_MOUSE | 59,551 | 5.1 |
| 66 | High | Peroxiredoxin-1 | PRDX1_MOUSE | 22,162 | 8.3 |
| 67 | High | Centrosomal protein C10orf90 homolog | CJ090_MOUSE | 71,476 | 8.8 |
| 68 | High | C2 calcium-dependent domain-containing protein 4D | C2C4D_MOUSE | 36,842 | 11.0 |
| 69 | Low | Tripartite motif-containing protein 43B | TR43B_MOUSE | 52,204 | 8.6 |
| 70 | High | Nucleoside diphosphate kinase B | NDKB_MOUSE | 17,352 | 7.0 |
| 78 | High | Histone H2A.Z | H2AZ_MOUSE | 13,545 | 10.6 |
| 79 | High | E3 ubiquitin-protein ligase RNF181 | RN181_MOUSE | 19,088 | 5.7 |
| 80 | Low | Replication factor C subunit 5 | RFC5_MOUSE | 38,072 | 7.7 |
All proteins were of murine origin, except for spot 30 (C4) and spot 47 (inter-alpha globulin inhibitor H4) which originated from Bos taurus. MW = molecular weight. pI = isoelectric point.